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KMID : 0861020180330060035
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Korea Journal of Herbology
2018 Volume.33 No. 6 p.35 ~ p.42
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Anti-inflammatory effects of Coptidis Rhizoma in chronic cold stress-exposed mice
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Choi Jin-Gyu
Huh Eu-Gene
Lee Won-Il
Kim Yun-Kyung
Lee Tae-Hee
Oh Myung-Sook
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Abstract
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Objectives : The aim of this study was to investigate whether the extract of Coptidis Rhizoma inhibits inflammation in chronic cold stress (CCS)-exposed mice or not.
Methods : Coptidis Rhizoma extract (CRE) was made by reflux with distilled water. Male ICR mice (7 weeks old) were divided randomly into 5 groups: (1) control, (2) CCS, (3) CCS+CRE 100 ㎎/㎏, (4) CCS+CRE 300 ㎎/㎏, (5) CCS+CRE 1,000 ㎎/㎏ groups. Mice were orally administered once a day for 14 days starting from 1 day before CCS. Group (2)-(5) were exposed to CCS conditions that maintained at 4℃ for 2 h once a day for 14 days. The levels of serum cortisol and hypothalamic prostaglandin E1 (PGE1) and PGE2 were measured by enzyme-linked immunosorbent assay kit. The expression levels of several pro-inflammatory factors like heat shock protein 70 (HSP70), c-fos, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) were measured by western blot analysis in mouse hypothalamus.
Results : Oral administration of CRE 1,000 ㎎/㎏ significantly suppressed the increase of serum cortisol levels in mice exposed to CCS. CCS-exposed mice had significantly increased the expression of HSP70, c-fos, and NF-kB in hypothalamus, while CRE treatment significantly attenuated the elevation of these pro-inflammatory factors. The ratio of PGE2/PGE1 was also higher in CCS-exposed mice than control group. CRE treatment significantly reduced the increase of PGE2/PGE1 ratio induced by CCS.
Conclusion : These findings suggest that Coptidis Rhizoma may work as a potential agent to modulate inflammatory responses under the condition of cold adaptation formed by CCS.
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KEYWORD
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Coptidis Rhizoma, chronic cold stress, inflammation
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